Keratin 10 antibody

Catalog No. ABIN6295934

The Mouse Monoclonal anti-Keratin 10 antibody has been validated for IHC, WB, FACS and IF. It is suitable to detect Keratin 10 in samples from Human and Cat.

Quick Overview for Keratin 10 antibody (ABIN6295934)

Target: Keratin 10 (KRT10)

Reactivity: Human, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 10 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-Keratin 10 Antibody

Purpose: Mouse anti-Human/Cat Cytokeratin 10 + 13 Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: A cytoskeletal preparation extracted from human ectocervical epithelium was used as the immunogen for the Cytokeratin 10 + 13 antibody.

Immunogen: A cytoskeletal preparation extracted from human ectocervical epithelium was used as the immunogen for the Cytokeratin 10 + 13 antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Western blot: 0.25-0.5 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Keratin 10

Target: Keratin 10 (KRT10)

Alternative Name: Keratin, type I cytoskeletal 10

Background:

Target Description: This antibody recognizes cytokeratin 10 (56.5 kDa) and cytokeratin 13 (53 kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this mAb serves as differentiation-related marker of all stratified epithelia, it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.

Gene Symbol: KRT10///KPP///KRT13

UniProt: P13645, P13646