MAML3 antibody

Catalog No. ABIN6295979

The Mouse Monoclonal anti-MAML3 antibody has been validated for FACS, IF and IHC. It is suitable to detect MAML3 in samples from Human.

Quick Overview for MAML3 antibody (ABIN6295979)

Target: MAML3 (Mastermind-Like 3 (MAML3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAML3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-MAML3 Antibody

Purpose: Mouse anti-Human MAML3 Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: A human full length recombinant protein was used as the immunogen for this MAML3 antibody.

Immunogen: A human full length recombinant protein was used as the immunogen for this MAML3 antibody.

Application Details

Application Notes: FACS: 0.5-1 μg/10^6 cells
IF: 1-2 μg/mL
IHC (FFPE): 0.5-1 μg/mL (1)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for MAML3

Target: MAML3 (Mastermind-Like 3 (MAML3))

Alternative Name: Mastermind-like protein 3

Background:

Target Description: Mastermind-like 3 is a nuclear speckle protein that acts as a transcriptional coactivator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML3 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML3 interacts with the ankyrin repeats of the Notch 1-4 proteins. This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-Jκ, a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML3 is essential for proper Notch binding, the C-terminal domain is essential for transcriptional activation. Due to its involvement in cell signaling and transcriptional activation, its upregulation is thought to be involved in oncogenesis.

Gene Symbol: MAML3///KIAA1816

Gene ID: 55534

UniProt: Q96JK9