Desmoglein 2 antibody

Catalog No. ABIN6296077

This Mouse Monoclonal antibody specifically detects Desmoglein 2 in WB, IF, FACS and IP. It exhibits reactivity toward Human.

Quick Overview for Desmoglein 2 antibody (ABIN6296077)

Target: Desmoglein 2 (DSG2)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Desmoglein 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP)

Product Details anti-Desmoglein 2 Antibody

Purpose: Mouse anti-Human Desmoglein 2 Antibody / DSG2 [Sodium Azide Free]

Specificity: Cell surface

Purification: A recombinant human DSG2 protein was used as the immunogen for the Desmoglein 2 antibody.

Immunogen: A recombinant human DSG2 protein was used as the immunogen for the Desmoglein 2 antibody.

Application Details

Application Notes: FACS: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
WB: 1-2 μg/mL
Immunoprecipitation: suitable

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Desmoglein 2

Target: Desmoglein 2 (DSG2)

Alternative Name: Desmoglein-2

Background:

Target Description: Recognizes a protein of 165 kDa, identified as Desmoglein-2 (DSG2). This monoclonal antibody recognizes the extracellular domain of human desmoglein-2. Desmoglein-2 is a member of the desmosomal cadherin family. Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. Desmocollins and desmogleins are the main desmosomal transmembrane proteins. Desmogleins consist of Dsg1, Dsg2, Dsg3, and Dsg4 isoforms. Within the desmosome, the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Human Desmoglein-2 is a type I transmembrane glycoprotein of 1117 amino acid (aa) residues with a 23 aa signal peptide and a 25 aa propeptide. It differs from other classic cadherins by having four instead of five cadherin repeat domains in its extracellular region, and a much larger cytoplasmic region containing five desmoglein repeat domains which share homology with the cadherin repeats. Instead of having the HAV adhesion motif found in type I cadherins, desmogleins have R/YAL as the adhesion motif on its amino-terminal cadherin repeat. The cytoplasmic tails of desmogleins interact with desmoplakins, plakoglobin and plakophilins. In turn, these proteins link the desmogleins with the intermediate filaments. Desmoglein-2 has been shown to be important in establishing cell-cell adhesion and function in epithelial cells. Desmoglein2 was originally identified in colon carcinoma and colon, and was named HDGC (human desmoglein colon).

Gene Symbol: DSG2///CDHF5

Gene ID: 1829

UniProt: Q14126

Pathways: Cell-Cell Junction Organization