WT1 antibody

Catalog No. ABIN6296102

The Rabbit Polyclonal anti-WT1 antibody has been validated for IHC, IF and FACS. It is suitable to detect WT1 in samples from Human.

Quick Overview for WT1 antibody (ABIN6296102)

Target: WT1 (Wilms Tumor 1 (WT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WT1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-WT1 Antibody

Purpose: Rabbit anti-Human Recombinant WT1 Antibody / Wilms Tumor 1 [Sodium Azide Free]

Specificity: Cytoplasmic, membranous

Purification: A recombinant protein was used as the immunogen for the recombinant WT1 antibody.

Immunogen: A recombinant protein was used as the immunogen for the recombinant WT1 antibody.

Application Details

Application Notes: FACS: 0.5-1 μg/10e6 cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT
Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Alternative Name: Wilms tumor protein

Background:

Target Description: Wilms tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilms tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.

Gene Symbol: WT1

Gene ID: 7490

UniProt: P19544

Pathways: Tube Formation