MUC5AC antibody

Catalog No. ABIN6296195

The Mouse Monoclonal anti-MUC5AC antibody has been validated for IHC, IF and FACS. It is suitable to detect MUC5AC in samples from Human, Mouse, Rat, Monkey, Cat, Rabbit, Chicken, Pig and Hedgehog.

Quick Overview for MUC5AC antibody (ABIN6296195)

Target: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Reactivity: Human, Mouse, Rat, Monkey, Cat, Rabbit, Chicken, Pig, Hedgehog

Host: Mouse

Clonality: Monoclonal

Conjugate: This MUC5AC antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-MUC5AC Antibody

Purpose: Mouse anti-Human/Monkey/Rabbit/Cat/Mouse/Rat/Pig/Hedgehog/Chicken Mucin-5AC Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: An M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient was used as the immunogen for this Mucin-5AC antibody.

Immunogen: An M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient was used as the immunogen for this Mucin-5AC antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 1-2 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for MUC5AC

Target: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Alternative Name: Mucin-5AC

Background:

Target Description: This mAb recognizes the peptide core of gastric mucin M1 (>1,000 kDa) (recently identified as Mucin 5AC). Its epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. Antibody to gastric mucin M1 reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.

Gene Symbol: MUC5AC///MUC5

Gene ID: 4586

UniProt: P98088