NUMA1 antibody

Catalog No. ABIN6296230

This Mouse Monoclonal antibody specifically detects NUMA1 in WB, IHC and IF. It exhibits reactivity toward Human.

Quick Overview for NUMA1 antibody (ABIN6296230)

Target: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NUMA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-NUMA1 Antibody

Purpose: Mouse anti-Human NuMA Antibody [Sodium Azide Free]

Specificity: Nuclear

Purification: Colon carcinoma 174T cells were used as the immunogen for the NuMA antibody.

Immunogen: Colon carcinoma 174T cells were used as the immunogen for the NuMA antibody.

Application Details

Application Notes: Western blot: 1-2 μg/mL
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for NUMA1

Target: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Alternative Name: Nuclear mitotic apparatus protein 1

Background:

Target Description: Recognizes a phosphorylated protein of 228 kDa, identified as nuclear mitotic apparatus protein (NuMA). Its epitope is resistant to phosphatases. NuMA is intra-nuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200 kDa during apoptosis. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia.

Gene Symbol: NUMA1///NMP22///NUMA

Gene ID: 4926

UniProt: Q14980

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, M Phase