CNN1 antibody

Catalog No. ABIN6296236

This Mouse Monoclonal antibody specifically detects CNN1 in FACS, IF and IHC. It exhibits reactivity toward Human and Rat.

Quick Overview for CNN1 antibody (ABIN6296236)

Target: CNN1 (Calponin 1 (CNN1))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CNN1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-CNN1 Antibody

Purpose: Mouse anti-Human/Rat Calponin Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: Crude human uterus extract was used as the immunogen for the Calponin antibody.

Immunogen: Crude human uterus extract was used as the immunogen for the Calponin antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for CNN1

Target: CNN1 (Calponin 1 (CNN1))

Alternative Name: Calponin-1

Background:

Target Description: Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist, a 34 kDa form and a 29 kDa form. Expression of the 29 kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this mAb reacts with only the 34 kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.

Gene Symbol: CNN1

Gene ID: 1264

UniProt: P51911