FOXP3 antibody

Catalog No. ABIN6296306

The Mouse Monoclonal anti-FOXP3 antibody has been validated for FACS and IF. It is suitable to detect FOXP3 in samples from Human, Mouse and Monkey.

Quick Overview for FOXP3 antibody (ABIN6296306)

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Reactivity: Human, Mouse, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This FOXP3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-FOXP3 Antibody

Purpose: Mouse anti-Human/Monkey/Mouse FOXP3 Antibody [Sodium Azide Free]

Specificity: Predominantly nuclear, some cytoplasmic

Purification: Full-length His-tagged mouse FOXP3 protein was used as the immunogen for this FOXP3 antibody.

Immunogen: Full-length His-tagged mouse FOXP3 protein was used as the immunogen for this FOXP3 antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence (0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for FOXP3

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Alternative Name: Forkhead box protein P3

Background:

Target Description: Recognizes a protein of 47-55 kDa, which is identified as FOXP3. Its precise epitope is not known, but it has been mapped to the N-terminal portion (amino acids 2-136) of the protein.The FOX family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. During early development, FOXP1 and FOXP2 are expressed abundantly in the lung, with lower levels of expression in neural, intestinal and cardiovascular tissues, where they act as transcription repressors. FOXP1 is widely expressed in adult tissues, while neoplastic cells often exhibit a dramatic change in expression level or localization of FOXP1. Mutations in FOXP3 gene cause IPEX, a fatal, X-linked inherited disorder characterized by immune dysregulation. The FOXP3 protein is essential for normal immune homeostasis. Specifically, FOXP3 represses transcription through a DNA binding forkhead domain, thereby regulating T cell activation.

Gene Symbol: FOXP3///IPEX///JM2

Gene ID: 50943

UniProt: Q9BZS1

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation