C4A antibody

Catalog No. ABIN6296522

The Mouse Monoclonal anti-C4A antibody has been validated for IHC, ELISA and IF. It is suitable to detect C4A in samples from Human.

Quick Overview for C4A antibody (ABIN6296522)

Target: C4A (C4a) (Complement Fragment C4a (C4a))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This C4A antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-C4A Antibody

Purpose: Mouse anti-Human C4d Antibody Complement 4d [Sodium Azide Free]

Specificity: Intracytoplasmic vacuoles of endothelial cells, Secreted

Purification: Human protein was used as the immunogen for this C4d antibody.

Immunogen: Human protein was used as the immunogen for this C4d antibody.

Application Details

Application Notes: IF: 1:50-1:100
IHC (FFPE): 1:200-1:400 for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2),ELISA: 1-2 μg/mL, order the BSA-free format

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C

Storage Comment: 2-8°C

Target Details for C4A

Target: C4A (C4a) (Complement Fragment C4a (C4a))

Alternative Name: Complement C4-A

Background:

Target Description: This antibody is specific to Complement 4d (C4d) and it reacts with the secreted as well as cell-bound protein. C4d is a degradation product of the activated complement factor C4b. Complement 4b is typically activated by binding of antibodies to specific target molecules. Following activation and degradation of the C4 Molecule, thio-ester groups are exposed, which allow transient, covalent binding of the degradation product C4d to endothelial cell surfaces and extracellular matrix components of vascular basement membranes near the sites of C4 activation. The presence of C4d in peritubular capillaries is a key indicator for acute humoral (i.e. antibody-mediated) rejection of kidney, heart, pancreas and lung allografts. As an established marker of antibody-mediated acute renal allograft rejection and its proclivity for endothelium, this component can be detected in peritubular capillaries in chronic renal allograft rejection as well as hyperacute rejection, acute vascular rejection, acute cellular rejection, and borderline rejection. C4d has been shown to be a significant predictor of transplant kidney graft survival. C4d antibody, combined with antibody to C3d, can be utilized as a tool for diagnosis of allograft rejection that may warrant a prompt and aggressive anti-rejection treatment.

Gene Symbol: C4A///CO4///CPAMD2///C4B///CO4///CPAMD3///C4B_2

Gene ID: 720

UniProt: P0C0L4, P0C0L5

Pathways: Complement System