CD55 antibody

Catalog No. ABIN6296784

This anti-CD55 antibody is a Mouse Monoclonal antibody detecting CD55 in FACS, IF and IHC (fro). Suitable for Human.

Quick Overview for CD55 antibody (ABIN6296784)

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD55 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CD55 Antibody

Purpose: Mouse anti-Human CD55 Antibody (DAF) [Sodium Azide Free]

Specificity: Cell surface

Purification: PHA stimulated human PBL were used as the immunogen for the CD55 antibody.

Immunogen: PHA stimulated human PBL were used as the immunogen for the CD55 antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (Frozen): 0.5-1 μg/mL for 30 min at RT

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for CD55

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Alternative Name: Complement decay-accelerating factor

Background:

Target Description: Recognizes a single chain glycoprotein of 70 kDa, identified as CD55 (also known as decay accelerating factor, DAF). CD55/DAF is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. The mAb 143-30 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH).

Gene Symbol: CD55///CR///DAF

Gene ID: 1604

UniProt: P08174

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity