SUMO2 antibody

Catalog No. ABIN6297478

This Mouse Monoclonal antibody specifically detects SUMO2 in IHC, IF and FACS. It exhibits reactivity toward Human and Rat.

Quick Overview for SUMO2 antibody (ABIN6297478)

Target: SUMO2 (Small Ubiquitin Related Modifier 2 (SUMO2))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SUMO2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-SUMO2 Antibody

Purpose: Mouse anti-Human/Rat SUMO-2 Antibody [Sodium Azide Free]

Specificity: Nuclear

Purification: Recombinant human protein was used as the immunogen for the SUMO-2 antibody.

Immunogen: Recombinant human protein was used as the immunogen for the SUMO-2 antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for SUMO2

Target: SUMO2 (Small Ubiquitin Related Modifier 2 (SUMO2))

Alternative Name: Small ubiquitin-related modifier 2

Background:

Target Description: The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, 2 and 3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2 and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1, 2 and 3 proteins localize to the nuclear membrane, nuclear bodies and cytoplasm, respectively. SUMO-1 utilizes Ubc9 for conjugation to several target proteins, which include MDM2, p53, PML and RanGap1. SUMO-2 and 3 contribute to a greater percentage of protein modification than does SUMO-1 and unlike SUMO-1, they can form polymeric chains. In addition, SUMO-3 regulates beta-Amyloid generation and may be critical in the onset or progression of Alzheimer s disease.

Gene Symbol: SUMO2///SMT3B///SMT3H2

Gene ID: 6613

UniProt: P61956

Pathways: Methionine Biosynthetic Process