PMEL antibody (Premelanosome Protein)

Details for Product anti-PMEL Antibody No. ABIN6297733
Target
Reactivity
Human
112
23
6
5
4
2
1
1
1
1
Host
Mouse
65
45
1
Clonality
Monoclonal
Conjugate
This PMEL antibody is un-conjugated
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3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
52
50
47
46
29
17
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5
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Options
Purpose Mouse anti-Human gp100 Antibody [Sodium Azide Free]
Immunogen The extract of pigmented melanoma metastases from lymph nodes was used as the immunogen for this gp100 antibody.
Specificity Cytoplasmic
Purification The extract of pigmented melanoma metastases from lymph nodes was used as the immunogen for this gp100 antibody.
Target
Alternative Name Melanocyte protein PMEL , M-beta] (PMEL Antibody Abstract)
Background

Target Description: Melanocytes produce organelles called melanosomes which produce melanin, the pigment that gives color to skin, hair, eyes, scales and feathers. gp100 was identified in an attempt to clone the gene Tyrosinase, an enzyme required for melanin synthesis. Further testing determined that gp100 is a melanoma-specific protein and is responsible for melanosome maturation, facilitating the transition from amorphous rounded vesicles to fibrillary ellipsoid organelles.
Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. Clone HMB45 gp100 antibody stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. It also stains angiomyolipomas, tumors most commonly associated with the kidney. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. This gp100 antibody does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Gene Symbol: PMEL///D12S53E///PMEL17///SILV

Gene ID 6490
UniProt P40967
Application Notes Western Blot: 1-2 μg/mL
FACS:0.5-1 μg/million cells
IF: 0.5-1 μg/mL
IHC (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Restrictions For Research Use only
Buffer In 1X PBS ( pH 7.4), BSA free, sodium azide free
Preservative Azide free
Storage 4 °C,-20 °C
Storage Comment 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.