Melanoma gp100 antibody (N-Term)

Catalog No. ABIN2782746

Cited in 1 publication. The Rabbit Polyclonal anti-Melanoma gp100 antibody (ABIN2782746) specifically detects Melanoma gp100 in IHC, WB and IP. The antibody is reactive with Human, Rat, Mouse, Horse, Dog, Cow, Goat, Guinea Pig and Rabbit samples.

Quick Overview for Melanoma gp100 antibody (N-Term) (ABIN2782746)

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Reactivity: Human, Rat, Mouse, Horse, Dog, Cow, Goat, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Melanoma gp100 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-Melanoma gp100 Antibody

Binding Specificity: N-Term

Purpose: SILV Antibody - N-terminal region

Sequence: HFLRNQPLTF ALQLHDPSGY LAEADLSYTW DFGDSSGTLI SRALVVTHTY

Predicted Reactivity: Cow: 93%, Dog: 86%, Goat: 93%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 86%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against SILV. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SILV

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8108878

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-Melanoma gp100 antibody (ABIN2782746)

Theos, Truschel, Tenza, Hurbain, Harper, Berson, Thomas, Raposo, Marks: "A lumenal domain-dependent pathway for sorting to intralumenal vesicles of multivesicular endosomes involved in organelle morphogenesis." in: Developmental cell, Vol. 10, Issue 3, pp. 343-54, (2006) (PubMed).

Target Details for Melanoma gp100

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Alternative Name: SILV

Background:

Background Information: SILV could be a melanogenic enzyme. It could represent an oncofetal self-antigen that is normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth. Release of the soluble form, ME20-S, it could protect tumor cells from antibody mediated immunity.

Gene Name: Premelanosome protein

Alternative Symbols: P1, SI, SIL, ME20, P100, SILV, ME20M, gp100, ME20-M, PMEL17, D12S53E

Protein Name: Melanocyte protein PMEL

Molecular Weight: 70kDa

Gene ID: 6490

NCBI Accession: NP_008859

UniProt: P40967