Histone H1 antibody (N-Term)

Catalog No. ABIN630685

This Rabbit Polyclonal antibody specifically detects Histone H1 in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Histone H1 antibody (N-Term) (ABIN630685)

Target: Histone H1 (H1F0) (H1 Histone Family, Member 0 (H1F0))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Histone H1 Antibody

Binding Specificity: N-Term

Specificity: H1 F0 antibody was raised against the N terminal of H1 0

Purification: Affinity purified

Immunogen: H1 F0 antibody was raised using the N terminal of H1 0 corresponding to a region with amino acids IQAEKNRAGSSRQSIQKYIKSHYKVGENADSQIKLSIKRLVTTGVLKQTK

Application Details

Application Notes: WB: 1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

H1F0 Blocking Peptide, (ABIN939492), is also available for use as a blocking control in assays to test for specificity of this H1F0 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Lyophilized powder. Add distilled water for a 1 mg/mL concentration of H0 0 antibody in PBS

Concentration: Lot specific

Buffer: PBS

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.

Target Details for Histone H1

Target: Histone H1 (H1F0) (H1 Histone Family, Member 0 (H1F0))

Alternative Name: H1F0

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. H1F0 gene is intronless and encodes a member of the histone H1 family.

Molecular Weight: 21 kDa (MW of target protein)