ATP6V1B2 antibody (N-Term)

Catalog No. ABIN631079

The Rabbit Polyclonal anti-ATP6V1B2 antibody has been validated for WB. It is suitable to detect ATP6V1B2 in samples from Human, Mouse and Rat.

Quick Overview for ATP6V1B2 antibody (N-Term) (ABIN631079)

Target: ATP6V1B2 (ATPase, H+ Transporting, Lysosomal 56/58kDa, V1 Subunit B2 (ATP6V1B2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP6V1B2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ATP6V1B2 Antibody

Binding Specificity: N-Term

Specificity: ATP6 V6 2 antibody was raised against the N terminal of ATP6 6 2

Purification: Affinity purified

Immunogen: ATP6 V6 2 antibody was raised using the N terminal of ATP6 6 2 corresponding to a region with amino acids VSRNYLSQPRLTYKTVSGVNGPLVILDHVKFPRYAEIVHLTLPDGTKRSG

Application Details

Application Notes: WB: 1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

ATP6V1B2 Blocking Peptide, (ABIN5612236), is also available for use as a blocking control in assays to test for specificity of this ATP6V1B2 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Lyophilized powder. Add distilled water for a 1 mg/mL concentration of ATP0 0 2 antibody in PBS

Concentration: Lot specific

Buffer: PBS

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.

Target Details for ATP6V1B2

Target: ATP6V1B2 (ATPase, H+ Transporting, Lysosomal 56/58kDa, V1 Subunit B2 (ATP6V1B2))

Alternative Name: ATP6V1B2

Background: ATP6V1B2 is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. ATP6V1B2 is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts.

Molecular Weight: 56 kDa (MW of target protein)

Pathways: Transition Metal Ion Homeostasis, Proton Transport