ube3a antibody (N-Term)

Catalog No. ABIN633810

This anti-ube3a antibody is a Rabbit Polyclonal antibody detecting ube3a in WB. Suitable for Human.

Quick Overview for ube3a antibody (N-Term) (ABIN633810)

Target: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ube3a antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ube3a Antibody

Binding Specificity: N-Term

Specificity: UBE3 A antibody was raised against the N terminal Of Ube3

Purification: Affinity purified

Immunogen: UBE3 A antibody was raised using the N terminal Of Ube3 corresponding to a region with amino acids SLQAKDEDKDEDEKEKAACSAAAMEEDSEASSSRIGDSSQGDNNLQKLGP

Application Details

Application Notes: WB: 1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

UBE3A Blocking Peptide, (ABIN5616878), is also available for use as a blocking control in assays to test for specificity of this UBE3A antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Lyophilized powder. Add distilled water for a 1 mg/mL concentration of UBE0 antibody in PBS

Concentration: Lot specific

Buffer: PBS

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.

Target Details for ube3a

Target: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Alternative Name: UBE3A

Background: UBE3A is an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53.

Molecular Weight: 101 kDa (MW of target protein)

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway