Doublecortin antibody (Internal Region)

Catalog No. ABIN6391363

This Goat Polyclonal antibody specifically detects Doublecortin in WB, ELISA, IHC and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for Doublecortin antibody (Internal Region) (ABIN6391363)

Target: Doublecortin (DCX)

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Doublecortin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Grade: Verified

Product Details anti-Doublecortin Antibody

Binding Specificity: Internal Region

Purpose: Doublecortex / DCX

Sequence: KASPTPQKTS AKSPG

Specificity: This antibody is expected to recognise isoform a (NP_000546.2), isoform b (NP_835365.1), and isoform c (NP_835364.1 and NP_835366.1).

Cross-Reactivity: Cat, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 7 μg/mL.

Western Blot: Approx 45 kDa band observed in Mouse fetal Brain lysates (calculated MW of 40.6 kDa according to Mouse NP_001103692.1). Recommended concentration: 0.003-0.01 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3 cells. Recommended concentration: 5μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Doublecortin

Target: Doublecortin (DCX)

Alternative Name: DCX

Background: DCX , doublecortex, lissencephaly, X-linked (doublecortin) , RP5-914P14.1, DBCN, DC, LISX, SCLH, XLIS , OTTHUMP00000062892, doublecortin, doublin, lissencephalin-X

Gene ID: 1641

NCBI Accession: NP_000546, NP_835365, NP_835364, NP_835366