CTCF antibody (Internal Region)

Catalog No. ABIN6391368

This anti-CTCF antibody is a Goat Polyclonal antibody detecting CTCF in WB and ELISA. Suitable for Human.

Quick Overview for CTCF antibody (Internal Region) (ABIN6391368)

Target: CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CTCF antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-CTCF Antibody

Binding Specificity: Internal Region

Purpose: CTCF (isoform 1)

Sequence: KKSKLRYTEE GKD

Specificity: This antibody is expected to recognize reported isoform 1 (NP_006556.1) only.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 150 kDa band observed in lysates of cell line Jurkat and nuclear Jurkat (calculated MW of 82.8 kDa according to NP_006556.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.03-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CTCF

Target: CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))

Alternative Name: CTCF

Background: CTCF, CCCTC-binding factor (zinc finger protein), 11 zinc finger transcriptional repressor, 11-zinc finger protein, CTCFL paralog, transcriptional repressor CTCF

Gene ID: 10664, 13018, 83726

NCBI Accession: NP_006556