CLEC2D antibody (Internal Region)

Catalog No. ABIN6391372

This Goat Polyclonal antibody specifically detects CLEC2D in ELISA, WB, IHC, FACS and IF. It exhibits reactivity toward Human.

Quick Overview for CLEC2D antibody (Internal Region) (ABIN6391372)

Target: CLEC2D (C-Type Lectin Domain Family 2, Member D (CLEC2D))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CLEC2D antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-CLEC2D Antibody

Binding Specificity: Internal Region

Purpose: OCIL

Sequence: LLRYKGPSDH

Specificity: This antibody is expected to recognize all isoforms (NP_037401.1, NP_001004419.1, NP_001184246.1).

Cross-Reactivity: Cow, Dog, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Tonsil and Placenta. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 30-35 kDa band observed in lysates of histiocytic lymphoma cell line U937 (calculated MW of 22.3 kDa according to NP_001004419.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1 μg/mL. Pr

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CLEC2D

Target: CLEC2D (C-Type Lectin Domain Family 2, Member D (CLEC2D))

Alternative Name: CLEC2D

Background: CLEC2D, C-type lectin domain family 2, member D, CLAX, LLT1, OCIL, C-type lectin related f, C-type lectin superfamily 2, member D, lectin-like NK cell receptor, lectin-like transcript 1, osteoclast inhibitory lectin

Gene ID: 29121

NCBI Accession: NP_037401, NP_001004419, NP_001184246

Pathways: Regulation of Leukocyte Mediated Immunity