PSME1 antibody (C-Term)

Catalog No. ABIN651448

This Rabbit Polyclonal antibody specifically detects PSME1 in WB, FACS and IHC (p). It exhibits reactivity toward Human.

Quick Overview for PSME1 antibody (C-Term) (ABIN651448)

Target: PSME1 (Proteasome (Prosome, Macropain) Activator Subunit 1 (PA28 Alpha) (PSME1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSME1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB21015

Product Details anti-PSME1 Antibody

Binding Specificity: AA 220-249, C-Term

Predicted Reactivity: B, Pr, M, Pig, Rat

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This PSME1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220-249 amino acids from the C-terminal region of human PSME1.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for PSME1

Target: PSME1 (Proteasome (Prosome, Macropain) Activator Subunit 1 (PA28 Alpha) (PSME1))

Alternative Name: PSME1

Background: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring.

Molecular Weight: 28723

Gene ID: 5720

NCBI Accession: NP_006254

UniProt: Q06323

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA