B3GALT5 antibody (N-Term)

Catalog No. ABIN655087

Product Details anti-B3GALT5 Antibody

Target: B3GALT5 (UDP-Gal:betaGlcNAc beta 1,3-Galactosyltransferase, Polypeptide 5 (B3GALT5))

Binding Specificity: AA 57-84, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This B3GALT5 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This B3GALT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-84 amino acids from the N-terminal region of human B3GALT5.

Clone: RB24267

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:1000. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for B3GALT5

Target: B3GALT5 (UDP-Gal:betaGlcNAc beta 1,3-Galactosyltransferase, Polypeptide 5 (B3GALT5))

Alternative Name: B3GALT5 (B3GALT5 Products)

Background: This gene is a member of the beta-1,3-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine). The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon. The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1, whereas beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1:type 2 chains changes during embryogenesis. By sequence similarity, the beta3GalT genes fall into at least two groups: beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3, beta3GalT5). This gene encodes the most probable candidate for synthesis of the type 1 Lewis antigens which are frequently found to be elevated in gastrointestinal and pancreatic cancers. The encoded protein is inactive with N-linked glycoproteins and functions in mucin glycosylation. Five transcript variants have been described which differ in the 5' UTR. All transcript variants encode an identical protein.

Molecular Weight: 36189

Gene ID: 10317

NCBI Accession: NP_001265579, NP_006048, NP_149360, NP_149361, NP_149362

UniProt: Q9Y2C3