RAF1 antibody (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1)

Details for Product anti-RAF1 Antibody No. ABIN6566510
Antigen
Reactivity
Human, Mouse (Murine), Rat (Rattus)
930
493
472
26
22
19
15
14
7
7
5
4
4
2
2
1
1
Host
Rabbit
887
41
6
6
2
Clonality
Polyclonal
Conjugate
This RAF1 antibody is un-conjugated
57
42
42
29
29
29
26
26
26
26
26
26
11
11
11
11
11
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
530
424
227
180
143
106
46
44
19
16
15
10
3
2
2
2
Options
Immunogen Recombinant protein of human RAF1
Isotype IgG
Purification Affinity purification
Antigen
Alternative Name RAF1 (RAF1 Antibody Abstract)
Background

Synonyms: c Raf,C-raf,C-Raf proto-oncogene,serine/threonine kinase,CMD1NN,Craf 1 transforming gene,cRaf,Craf1 transforming gene,EC 2.7.11.1,kinase Raf1,Murine sarcoma 3611 oncogene 1,NS5,Oncogene MIL,Oncogene RAF1,OTTHUMP00000160218,OTTHUMP00000207813,OTTHUMP00000209389,Protein kinase raf 1,Proto-oncogene c-RAF,Raf 1,Raf 1 proto oncogene serine/threonine kinase,RAF,Raf proto oncogene serine/threonine protein kinase,RAF proto-oncogene serine/threonine-protein kinase,RAF-1,RAF1,RAF1,Similar to murine leukemia viral (V-raf-1) oncogene homolog 1,TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV,v raf 1 murine leukemia viral oncogene homolog 1,v-raf murine sarcoma viral oncogene homolog 1,v-raf-1 murine leukemia viral oncogene-like protein 1,vraf1 murine leukemia viral oncogene homolog 1

Background: This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

Molecular Weight

Observed_MW: 75kDa

Calculated_MW: 73kDa/75kDa

Gene ID 5894
UniProt P04049
Pathways MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling
Application Notes WB 1:500 - 1:2000 IHC 1:50 - 1:200
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Buffer: PBS with 0.02 % sodium azide, 50 % glycerol,  pH 7.3.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze / thaw cycles.
Supplier Images
Image no. 1 for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody (ABIN6566510) Western blot analysis of extracts of various cell lines, using RAF1 antibody.
Image no. 2 for anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibody (ABIN6566510) Immunohistochemistry of paraffin-embedded rat liver using RAF1 antibody.