RAN antibody (RAN, Member RAS Oncogene Family)

Details for Product anti-RAN Antibody No. ABIN6566716
Antigen
Reactivity
Human, Monkey, Mouse (Murine)
159
119
101
8
8
6
5
4
4
3
3
3
2
2
2
1
1
1
Host
Rabbit
137
14
8
Clonality
Polyclonal
Conjugate
This RAN antibody is un-conjugated
9
6
6
5
5
5
5
5
5
4
4
4
2
2
2
2
2
Application
Immunofluorescence (IF), Western Blotting (WB)
118
70
61
30
26
23
11
10
9
1
1
1
1
Options
Immunogen Recombinant protein of human RAN
Isotype IgG
Purification Affinity purification
Antigen
Alternative Name RAN (RAN Antibody Abstract)
Background

Synonyms: Androgen receptor associated protein 24,Androgen receptor-associated protein 24,ARA 24,ARA24,Gsp1,GTP binding nuclear protein RAN,GTP-binding nuclear protein Ran,GTPase Ran,Guanosine triphosphatase Ran,LPS,OK/SW-cl.81,ran,RAN member RAS oncogene family,RAN,RanGTPase,Ras like protein TC4,Ras related nuclear protein,Ras-like protein TC4,Ras-related nuclear protein,RASL2 8,TC 4,TC4

Background: RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease.

Molecular Weight

Observed_MW: 23kDa

Calculated_MW: 24kDa

Gene ID 5901
UniProt P62826
Pathways Regulatory RNA Pathways, Intracellular Steroid Hormone Receptor Signaling Pathway, Protein targeting to Nucleus
Application Notes WB 1:500 - 1:2000 IF 1:50 - 1:200
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Buffer: PBS with 0.02 % sodium azide, 50 % glycerol,  pH 7.3.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze / thaw cycles.
Supplier Images
Image no. 1 for anti-RAN, Member RAS Oncogene Family (RAN) antibody (ABIN6566716) Western blot analysis of extracts of various cell lines, using RAN antibody.
Image no. 2 for anti-RAN, Member RAS Oncogene Family (RAN) antibody (ABIN6566716) Immunofluorescence analysis of HeLa cells using RAN antibody.