Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) antibody

Details for Product No. ABIN6566902
Antigen
Reactivity
Human, Mouse (Murine), Rat (Rattus)
108
55
50
10
10
5
4
3
3
2
1
1
1
1
1
1
1
1
Host
Rabbit
113
7
Clonality
Polyclonal
Conjugate
Un-conjugated
7
5
5
4
4
4
3
3
3
3
3
3
1
1
1
1
1
Application
Western Blotting (WB)
95
49
37
24
20
19
13
11
11
2
2
1
1
Options
Immunogen Recombinant protein of human GCLC
Isotype IgG
Purification Affinity purification
Antigen
Alternative Name GCLC (GCLC Antibody Abstract)
Background

Synonyms: EC 6.3.2.2,Gamma ECS,Gamma glutamylcysteine synthetase,Gamma-ECS,Gamma-glutamylcysteine synthetase,GCL,Gclc,GCS,GCS heavy chain,GLCL,GLCLC,Glutamate cysteine ligase catalytic subunit,Glutamate--cysteine ligase catalytic subunit,GSH1

Background: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.

Molecular Weight

Observed_MW: 72kDa

Calculated_MW: 72kDa

Gene ID 2729
UniProt P48506
Pathways Cell RedoxHomeostasis
Application Notes WB 1:500 - 1:2000
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Buffer: PBS with 0.02 % sodium azide, 50 % glycerol,  pH 7.3.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze / thaw cycles.
Supplier Images
Image no. 1 for anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) antibody (ABIN6566902) Western blot analysis of extracts of various cell lines, using GCLC antibody.