GCLC antibody (Middle Region)

Catalog No. ABIN2785780

This Rabbit Polyclonal antibody specifically detects GCLC in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Rabbit, Guinea Pig, Dog, Horse and Saccharomyces cerevisiae. It has been mentioned in 2+ publications

Quick Overview for GCLC antibody (Middle Region) (ABIN2785780)

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Rabbit, Guinea Pig, Dog, Horse, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCLC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GCLC Antibody

Binding Specificity: Middle Region

Purpose: GCLC Antibody - middle region

Sequence: RISKSRYDSI DSYLSKCGEK YNDIDLTIDK EIYEQLLQEG IDHLLAQHVA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against GCLC. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human GCLC

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP54577-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-GCLC antibody (ABIN2785780)

Ramani, Tomasi, Yang, Ko, Lu: "Mechanism and significance of changes in glutamate-cysteine ligase expression during hepatic fibrogenesis." in: The Journal of biological chemistry, Vol. 287, Issue 43, pp. 36341-55, (2012) (PubMed).

Bardag-Gorce, Oliva, Lin, Li, French, French: "Proteasome inhibitor up regulates liver antioxidative enzymes in rat model of alcoholic liver disease." in: Experimental and molecular pathology, Vol. 90, Issue 1, pp. 123-30, (2011) (PubMed).

Target Details for GCLC

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Alternative Name: GCLC

Background:

Background Information: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. GCLC is the catalytic subunit of 637 amino acids with a calculated molecular weight of 72.773 kDa. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.

Gene Name: Glutamate-cysteine ligase, catalytic subunit

Alternative Symbols: GCL, GCS, GLCL, GLCLC

Protein Name: Glutamate--cysteine ligase catalytic subunit

Molecular Weight: 73 kDa

Gene ID: 2729

NCBI Accession: NP_001489

UniProt: P48506

Pathways: Cell RedoxHomeostasis