Importin 5 antibody

Catalog No. ABIN6569027

This Rabbit Polyclonal antibody specifically detects Importin 5 in WB and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for Importin 5 antibody (ABIN6569027)

Target: Importin 5 (IPO5)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Importin 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-Importin 5 Antibody

Purification: Affinity purification

Immunogen: Recombinant fusion protein of human IPO5 (NP_002262.3).

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IF 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for Importin 5

Target: Importin 5 (IPO5)

Alternative Name: IPO5

Background: Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family.

Molecular Weight:

Observed_MW: 125kDa

Calculated_MW: 116kDa/123kDa/125kDa

Gene ID: 3843

UniProt: O00410

Pathways: Protein targeting to Nucleus