CDC34 antibody (N-Term)

Catalog No. ABIN190280

The Mouse Monoclonal anti-CDC34 antibody has been validated for WB, IHC (p), IP and IF/ICC. It is suitable to detect CDC34 in samples from Human.

Quick Overview for CDC34 antibody (N-Term) (ABIN190280)

Target: CDC34 (Cell Division Cycle 34 (CDC34))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDC34 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunofluorescence (fixed cells) (IF/ICC)

Clone: 14B5

Product Details anti-CDC34 Antibody

Binding Specificity: N-Term

Purpose: Anti-cdc34 Mouse Monoclonal Antibody

Specificity: This antibody recognizes human Cdc34.

Cross-Reactivity: Human

Immunogen: N-terminus 6x-His tagged fusion protein encoding full-length human Cdc34 expressed in E. coli.

Isotype: IgG1

Application Details

Application Notes: Immunoblotting and Immuno-precipitation: use at 1-5 μg/mL.
Positive controls: 293 cells and recombinant fusion protein.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium which is identical to that used in the assay system.

Concentration: Lot specific

Buffer: 10 mM PBS, pH 7.4.

Storage: -20 °C

Storage Comment: This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Target Details for CDC34

Target: CDC34 (Cell Division Cycle 34 (CDC34))

Alternative Name: CDC34

Background: Ubiquitin-conjugating enzyme E2 R1,Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination (PubMed:22496338). Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes (PubMed:10329681, PubMed:10373550, PubMed:10871850, PubMed:11675391, PubMed:12037680, PubMed:15652359, PubMed:17461777, PubMed:17698585, PubMed:19112177, PubMed:19126550, PubMed:19945379, PubMed:20061386, PubMed:20347421). {PubMed:10329681, PubMed:10373550, PubMed:10871850, PubMed:11675391, PubMed:12037680, PubMed:15652359, PubMed:17461777, PubMed:17698585, PubMed:19112177, PubMed:19126550, PubMed:19945379, PubMed:20061386, PubMed:20347421, PubMed:22496338}.,Cdc 34 is a ubiquitin conjugating enzyme that is essential for the transition from G1 to S phase in Saccharomyces cerevisiae. Human Cdc34 fully complements the budding yeast Cdc34 temperature sensitive mutant phenotype. In extracts of Xenopus laevis eggs, Cdc34 is required for the onset of DNA replication and appears to function in a multi-protein complex. Cdc34 is highly conserved among vertebrates and probably functions similarly in all eukaryotes to regulate the start of DNA synthesis.,Cytoplasm. Nucleus. Note=The phosphorylation of the C-terminal tail plays an important role in mediating nuclear localization. Colocalizes with beta-tubulin on mitotic spindles in anaphase.,EC 2.3.2.23,

UniProt: P49427