TSG101 antibody

Catalog No. ABIN190317

This anti-TSG101 antibody is a Mouse Monoclonal antibody detecting TSG101 in WB, IP, IF/ICC, PLA and BP. Suitable for Human.

Quick Overview for TSG101 antibody (ABIN190317)

Target: TSG101 (Tumor Susceptibility Gene 101 (TSG101))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TSG101 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (fixed cells) (IF/ICC), Proximity Ligation Assay (PLA), Blocking Peptide (BP)

Clone: 1F3

Product Details anti-TSG101 Antibody

Purpose: Anti-DNA Ligase III Mouse Monoclonal Antibody

Specificity: This antibody recognizes human, mouse, and chicken DNA Ligase III. Cellular localization is nuclear and mitochondrial.

Cross-Reactivity: Chicken, Human, Mouse

Immunogen: Full-length recombinant human DNA Ligase III-b.

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: use at 0.5-2 μg/mL. Predicted molecular weight ~100 kDa.
These are recommended concentrations.
Endusers should determine optimal concentrations for their applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium which is identical to that used in the assay system.

Concentration: 1.0 mg/mL

Buffer: Hybridoma culture supernatant

Storage: -20 °C

Storage Comment: This antibody is stable for at least one year at -20°C. Store in appropriate aliquots to avoid multiple freeze-thaw cycles.

Target Details for TSG101

Target: TSG101 (Tumor Susceptibility Gene 101 (TSG101))

Alternative Name: TSG101

Background: DNA ligase 3,Isoform 3 functions as heterodimer with DNA-repair protein XRCC1 in the nucleus and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Isoform 1 is targeted to mitochondria, where it functions as DNA ligase in mitochondrial base-excision DNA repair (PubMed:10207110, PubMed:24674627). {PubMed:10207110, PubMed:24674627}.,There are four distinct ligase proteins found in mammalian cells. These DNA ligases are required for joining DNA strand breaks that occur during DNA replication, repair and recombination. DNA Ligase III is a 100 kDa (a-form) protein in somatic tissues, is ubiquitously expressed, and complexes with XRCC-1 and a 97 kDa ( -form) protein that is found in testis. DNA Ligase III is involved in rejoining DNA single-strand breaks and in base excision repair pathway.,[Isoform 1]: Mitochondrion,EC 6.5.1.1, DNA ligase III, Polydeoxyribonucleotide synthase [ATP] 3

UniProt: P49916