CD5 antibody (AA 25-495)

Catalog No. ABIN6654154

The Rabbit Polyclonal anti-CD5 antibody is suitable to detect CD5 in samples from Human and Mouse. It has been validated for FACS, WB, ELISA and IHC (p).

Quick Overview for CD5 antibody (AA 25-495) (ABIN6654154)

Target: CD5

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD5 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CD5 Antibody

Binding Specificity: AA 25-495

Purification: Antigen affinity purified

Immunogen: Human CD5 recombinant protein (amino acids R25-L495) was used as the immunogen for the CD5 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the CD5 antibody should be determined by the researcher.\. Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL,Direct ELISA: 0.1-0.5 μg/mL (human recombinant protein),FACS: 1-3 μg/10^6 cells

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the CD5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for CD5

Target: CD5

Alternative Name: CD5

Background: CD5 is a member of the scavenger receptor cysteine-rich (SRCR) superfamily. Members of this family are secreted or membrane-anchored proteins mainly found in cells associated with the immune system. In humans, the gene is located on the long arm of chromosome 11. This protein is a type-I transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes and a subset of B lymphocytes. The encoded protein contains three SRCR domains and may act as a receptor to regulate T-cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt: P06127