ATRX antibody

Catalog No. ABIN6654951

This Rabbit Monoclonal antibody specifically detects ATRX in IHC (p). It exhibits reactivity toward Human.

Quick Overview for ATRX antibody (ABIN6654951)

Target: ATRX (helicase 2, X-linked (ATRX))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ATRX antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: ATMRX-1R

Product Details anti-ATRX Antibody

Purification: Purified

Purity: Protein A affinity chromatography

Immunogen: Recombinant human ATRX protein was used as the immunogen for this recombinant ATRX antibody.

Isotype: IgG kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant ATRX antibody to be titered up or down for optimal performance.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. IHC (FFPE): 1-2 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant ATRX antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for ATRX

Target: ATRX (helicase 2, X-linked (ATRX))

Alternative Name: ATRX / RAD54

Background: The protein encoded by this gene contains an ATPase/helicase domain, and thus it belongs to the SWI/SNF family of chromatin remodeling proteins. This protein is found to undergo cell cycle-dependent phosphorylation, which regulates its nuclear matrix and chromatin association, and suggests its involvement in the gene regulation at interphase and chromosomal segregation in mitosis. Mutations in this gene are associated with X-linked syndromes exhibiting cognitive disabilities as well as alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported. [RefSeq]