NEFH antibody

Catalog No. ABIN6655041

Supplier Product No.: 200-901-d82

This Chicken Polyclonal antibody specifically detects NEFH in WB, IF and FM. It exhibits reactivity toward Cow.

Quick Overview for NEFH antibody (ABIN6655041)

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reactivity: Cow

Host: Chicken

Clonality: Polyclonal

Conjugate: This NEFH antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Product Details anti-NEFH Antibody

Supplier Product No.: 200-901-d82

Supplier: Rockland

Purpose: Neurofilament H Antibody

Cross-Reactivity (Details): Anti-NF-H antibody is directed against NF-H.

Purification: The antibody is a total IgY fraction.

Immunogen: Anti-Neurofilament NF-H Antibody was produced by repeated immunizations with purified bovine NF-H.

Isotype: IgG

Application Details

Application Notes:

IF_Microscopy_Dilution: 1:25000

Western_Blot_Dilution: 1:50000

Other: User Optimized

Comment:

Anti-Neurofilament H antibody is tested for use in Western blotting and IF. Expect a band of approximately 200 kDa in size corresponding to the NF-H proteins in Western blots in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Alternative Name: Neurofilament H

Background:

Synonyms: Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein

Background: NF-H antibody detects Neurofilament H. Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease. Anti-Neurofilament H antibody is ideal for investigators involved in Cell Signaling, and Neuroscience research.

Gene Name: NEFH

Gene ID: 4744

UniProt: P12036