TIMP1 antibody (C-Term)

Catalog No. ABIN6655182

Supplier Product No.: 600-401-p97

This Rabbit Polyclonal antibody specifically detects TIMP1 in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for TIMP1 antibody (C-Term) (ABIN6655182)

Target: TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TIMP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-TIMP1 Antibody

Binding Specificity: C-Term

Supplier Product No.: 600-401-p97

Supplier: Rockland

Purpose: TIMP1 Antibody

Cross-Reactivity (Details): Anti-TIMP1 antibody is directed against human TIMP1 protein.

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: TIMP1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a sequence at the C-terminal of human TIMP1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:100-1:500

Western_Blot_Dilution: 0.5 μg/mL

Comment:

Anti-TIMP1 is tested for Immunohistochemistry -P and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~23.2kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide

Stabilizer: 5 mg BSA

Preservative: 0.05 mg Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for TIMP1

Target: TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))

Alternative Name: TIMP1

Background:

Synonyms: Erythroid-potentiating activity

Background: TIMP metallopeptidase inhibitor 1, also known as TIMP1, a tissue inhibitor of metalloproteinases, is a glycoprotein that is expressed from the several tissues of organisms. This protein a member of the TIMP family. The glycoprotein is a natural inhibitor of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. TIMP was found to be located about 22 cM proximal to OTC (300461). This antibody is suitable for researchers interested in extracellular matrix antibodies and cancer research.

Gene Name: TIMP1

Gene ID: 7076

NCBI Accession: NP_003245

UniProt: P01033