HIST1H2AB antibody (C-Term)

Catalog No. ABIN6655250

Supplier Product No.: 600-401-x48

This anti-HIST1H2AB antibody is a Rabbit Polyclonal antibody detecting HIST1H2AB in WB, ELISA, IF, ChIP and FM. Suitable for Human.

Quick Overview for HIST1H2AB antibody (C-Term) (ABIN6655250)

Target: HIST1H2AB (Histone Cluster 1, H2ab (HIST1H2AB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIST1H2AB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM)

Product Details anti-HIST1H2AB Antibody

Binding Specificity: C-Term

Supplier Product No.: 600-401-x48

Supplier: Rockland

Purpose: Histone H2A pan Antibody

Cross-Reactivity (Details): Cross reactivity with other species was not tested.

Purification: Anti-Histone H2A pan Antibody was purified by affinity purification.

Sterility: Sterile filtered

Immunogen: Anti-Histone H2A pan Antibody was produced in rabbits by repeated immunizations with a synthetic peptide containing a sequence from the C-terminus of histone H2A.

Isotype: IgG

Application Details

Application Notes:

ChIP_Dilution: 1-2 μg per IP

ELISA_Dilution: 1:100 - 1:1,000

IF_Microscopy_Dilution: 1:500

Western_Blot_Dilution: 1:2,000

Other: Dot Blot: 1:20,000

Comment:

Anti-Histone H2A pan Antibody has been tested by Chromatin Immunoprecipitation, western blot, immunofluorescence, and ELISA. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.05 % (w/v) Sodium Azide and 0.05 % ProClin 300

Preservative: ProClin, Sodium azide

Precaution of Use: This product contains Sodium azide and ProClin: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for HIST1H2AB

Target: HIST1H2AB (Histone Cluster 1, H2ab (HIST1H2AB))

Alternative Name: HIST1H2AB

Background:

Synonyms: Histone H2A type 1-B/E, Histone H2A.2, Histone H2A/a, Histone H2A/m, H2AFM, HIST1H2AE, H2AFA

Background: Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histones play a internal role in the regulation of transcription, DNA repair, DNA replication and chromosomal stability. These different functions are established via a complex set of post-translational modifications which either directly or indirectly alter chromatin structure and DNA accessibility to facilitate transcriptional activation or repression or other nuclear processes. Anti-Histone H2A pan Antibody is ideal for research in Chromatin Remodeling, Gene Expression and Epigenetics.

Gene Name: HIST1H2AB

Gene ID: 8329

NCBI Accession: NP_003500

UniProt: P0C0S8

Pathways: Telomere Maintenance