CBFB antibody (Internal Region)

Catalog No. ABIN6655308

Supplier Product No.: 100-401-v32

This Rabbit Polyclonal antibody specifically detects CBFB in ELISA and ChIP. It exhibits reactivity toward Human.

Quick Overview for CBFB antibody (Internal Region) (ABIN6655308)

Target: CBFB (Core-Binding Factor, beta Subunit (CBFB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CBFB antibody is un-conjugated

Application: ELISA, Chromatin Immunoprecipitation (ChIP)

Product Details anti-CBFB Antibody

Binding Specificity: Internal Region

Supplier Product No.: 100-401-v32

Supplier: Rockland

Purpose: CBFb Antibody

Cross-Reactivity (Details): This product reacts with human CBFb.

Purification: Anti-CBFb Antibody is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration.

Sterility: Sterile filtered

Immunogen: Anti-CBFb Antibody was produced in rabbits by repeated immunizations with human CBFb using two synthetic peptides containing sequences from the internal region of the protein.

Isotype: IgG1

Application Details

Application Notes:

ChIP_Dilution: 4 μL/ChIP

ELISA_Dilution: 1:500

Comment:

Anti-CBFb Antibody has been tested in ChIP and ELISA. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: None

Stabilizer: None

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CBFB

Target: CBFB (Core-Binding Factor, beta Subunit (CBFB))

Alternative Name: CBFb

Background:

Synonyms: Core-binding factor subunit beta, CBF-beta, Polyomavirus enhancer-binding protein 2 beta subunit, PEA2-beta, PEBP2-beta, SL3-3 enhancer factor 1 subunit beta, SL3/AKV core-binding factor beta subunit

Background: CBFb represents the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family. These transcription factors regulate a host of genes specific to haematopoiesis (e.g. RUNX1) and osteogenesis (e.g. RUNX2). The beta subunit is the regulatory subunit which allosterically enhances the activity of the DNA binding alpha subunit as the complex binds to the core site of various enhancers and promoters. CBFb can be involved in a chromosomal rearrangement of chromosome 16 (inv(16)(p13q22)) which produces a fusion protein consisting of the N terminus of CBFb and the C-terminal portion of MYH11. This chromosomal rearrangement is associated with acute myeloid leukaemia of the M4Eo subtype. Anti-CBFb Antibody is ideal for research in Gene Expression, Cell Differentiation and Cancer.

Gene Name: CBFB

Gene ID: 865

NCBI Accession: NP_001746

UniProt: Q13951

Pathways: Regulation of Lipid Metabolism by PPARalpha