CD9 antibody (FITC)

Catalog No. ABIN6655324

Supplier Product No.: 200-302-n70

The Mouse Monoclonal anti-CD9 antibody has been validated for FACS, WB and IHC. It is suitable to detect CD9 in samples from Human.

Quick Overview for CD9 antibody (FITC) (ABIN6655324)

Target: CD9

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD9 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC)

Clone: HI100

Product Details anti-CD9 Antibody (FITC)

Supplier Product No.: 200-302-n70

Supplier: Rockland

Purpose: CD45RA Fluorescein Antibody

Cross-Reactivity (Details): Reactivity is observed against human CD45RA.

Purification: Fluorescein conjugated CD45RA Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD45RA.

Sterility: Sterile filtered

Immunogen: Anti-CD45RA Antibody (Monoclonal) was produced by repeated immunizations with CD45RA antigen.

Isotype: IgG2b kappa

Labeling Ratio: 2-8

Application Details

Application Notes:

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 5 uL/test/1x10e5 to 1x10e8 cells

Western_Blot_Dilution: User Optimized

Comment:

Anti-CD45RA is tested for Flow Cytometry and is useful in Immunohistochemistry and Western Blot. Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

Expiry Date: 6 months

Target Details for CD9

Target: CD9

Alternative Name: CD9

Background:

Synonyms: CD9 antigen, 5H9 antigen, Cell growth-inhibiting gene 2 protein, Leukocyte antigen MIC3, Motility-related protein, MRP-1, Tetraspanin-29, Tspan-29, p24, CD9, MIC3, TSPAN29, GIG2

Background: The HI100 antibody reacts with the human CD45 isoform known as CD45RA, a protein tyrosine phosphatase of 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns. With their broad cell distribution, CD45 isoforms are critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RB, CD45RO and others.The HI100 antibody is widely used as a marker for human CD45RA expression on naive and activated T cells, B cells, and monocytes.

Gene Name: CD9

Gene ID: 928

NCBI Accession: NP_001760

UniProt: P21926

Pathways: Response to Water Deprivation, Cell-Cell Junction Organization