KDM4A antibody (Internal Region)

Catalog No. ABIN6655514

Supplier Product No.: 600-401-x45

This anti-KDM4A antibody is a Rabbit Polyclonal antibody detecting KDM4A in WB and ELISA. Suitable for Human.

Quick Overview for KDM4A antibody (Internal Region) (ABIN6655514)

Target: KDM4A (Lysine (K)-Specific Demethylase 4A (KDM4A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KDM4A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-KDM4A Antibody

Binding Specificity: Internal Region

Supplier Product No.: 600-401-x45

Supplier: Rockland

Purpose: JMJD2A Antibody

Cross-Reactivity (Details): Cross reactivity with other species was not tested.

Purification: Anti-JMJD2a Antibody was purified by affinity purification.

Sterility: Sterile filtered

Immunogen: Anti-JMJD2a Antibody was produced in rabbits by repeated immunizations with a synthetic peptide from human JMJD2a containing a sequence from the internal part of the protein.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:100 - 1:500

Western_Blot_Dilution: 1:500

Comment:

Anti-JMJD2a Antibody is tested for ELISA and Western Blots. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 121 kDa in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.05 % (w/v) Sodium Azide and 0.05 % ProClin 300

Preservative: ProClin, Sodium azide

Precaution of Use: This product contains Sodium azide and ProClin: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for KDM4A

Target: KDM4A (Lysine (K)-Specific Demethylase 4A (KDM4A))

Alternative Name: JMJD2a

Background:

Synonyms: Lysine-specific demethylase 4A, JmjC domain-containing histone demethylation protein 3A, Jumonji domain-containing protein 2A, JHDM3A, JMJD2, KIAA0677

Background: JMJD2a, belongs to the JMJD2 family of histone demethylases which play an important role in the establishment of the histone code. JMJD2a specifically demethylates the trimethylated K9 and K36 of histone H3, thereby converting these lysines to the dimethylated form. It has no activity towards H3K4, H3K27 and H4K20, or to the mono- and dimethylated H3K9 and H3K36. JMJD2a plays a role in the transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. Anti-JMJD2a Antibody is ideal for research in Chromatin Remodeling, Gene Expression and Epigenetics.

Gene Name: KDM4A

Gene ID: 9682

NCBI Accession: NP_055478

UniProt: O75164

Pathways: Warburg Effect