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Immunoglobulin kappa Variable 1-27 (IGKV1-27) (C-Term) antibody Primary Antibody

IGKV1-27 Reactivity: Human IP, WB Host: Mouse Monoclonal 59A426
Catalog No. ABIN6655643
$411.00
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100 μg
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  • Target
    Binding Specificity
    C-Term
    Reactivity
    Human
    Host
    Mouse
    Clonality
    Monoclonal
    Application
    Immunoprecipitation (IP), Western Blotting (WB)
    Purification
    Anti-A20 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-A20 from Human, Mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-A20 from other sources has not been determined.
    Immunogen

    Immunogen: A20 Antibody was produced in mice by repeated immunizations with recombinant human A20 protein at the c-terminus.

    Immunogen Type: Recombinant Protein

    Clone
    59A426
    Isotype
    IgG1
  • Application Notes

    Application Note: Anti-A20 antibody is suitable for use in Flow (I), IF/ICC, IHC (p), IP and WB. Expect a band approximately 90 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

    Immunoprecipitation Dilution: 1-2 μg/mL

    Western Blot Dilution: 2-4 μg/mL

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 0.05 % BSA

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
  • Target
    Alternative Name
    A20 (IGKV1-27 Antibody Abstract)
    Background

    Synonyms: OTUD7C, Tumor necrosis factor alpha-induced protein 3, TNF alpha-induced protein 3, OTU domain-containing protein 7C, A20p50, A20p37

    Background: Anti-A20 antibody detects human A20. A20 (TNFAIP3) is an important regulator of proinflammatory signaling pathways, including NK-kB sginaling. A20 is upregulated through NF-kB (p50/p55) activation, and subsequently down regulates NF-kB through its dual function as a deubiquitinase and ubiquitin ligase. The A20 monoclonal antibody has been instrumental in discovering various nuances of NF-kB regulation and key to defining A20 biology. Anti-A20 antibody is ideal for investigators involved in NFkappaB and Signaling research.

    Gene Name: TNFAIP3

    Gene ID
    7128
    NCBI Accession
    NP_001257436
    UniProt
    P21580
    Pathways
    Activation of Innate immune Response
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