IGKV1-27 antibody (C-Term)

Catalog No. ABIN6655643

Product Details anti-IGKV1-27 Antibody

Target: IGKV1-27 (Immunoglobulin kappa Variable 1-27 (IGKV1-27))

Binding Specificity: C-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IGKV1-27 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Purification: Anti-A20 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-A20 from Human, Mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-A20 from other sources has not been determined.

Immunogen:

Immunogen: A20 Antibody was produced in mice by repeated immunizations with recombinant human A20 protein at the c-terminus.

Immunogen Type: Recombinant Protein

Clone: 59A426

Isotype: IgG1

Application Details

Application Notes:

Application Note: Anti-A20 antibody is suitable for use in Flow (I), IF/ICC, IHC (p), IP and WB. Expect a band approximately 90 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Immunoprecipitation Dilution: 1-2 μg/mL

Western Blot Dilution: 2-4 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for IGKV1-27

Target: IGKV1-27 (Immunoglobulin kappa Variable 1-27 (IGKV1-27))

Alternative Name: A20 (IGKV1-27 Products)

Synonyms: A20 antibody, OTUD7C antibody, TNFA1P2 antibody, TNF alpha induced protein 3 antibody, TNFAIP3 antibody

Background:

Synonyms: OTUD7C, Tumor necrosis factor alpha-induced protein 3, TNF alpha-induced protein 3, OTU domain-containing protein 7C, A20p50, A20p37

Background: Anti-A20 antibody detects human A20. A20 (TNFAIP3) is an important regulator of proinflammatory signaling pathways, including NK-kB sginaling. A20 is upregulated through NF-kB (p50/p55) activation, and subsequently down regulates NF-kB through its dual function as a deubiquitinase and ubiquitin ligase. The A20 monoclonal antibody has been instrumental in discovering various nuances of NF-kB regulation and key to defining A20 biology. Anti-A20 antibody is ideal for investigators involved in NFkappaB and Signaling research.

Gene Name: TNFAIP3

Gene ID: 7128

NCBI Accession: NP_001257436

UniProt: P21580

Pathways: Activation of Innate immune Response