Caspase 1 antibody (C-Term)

Catalog No. ABIN6655699

Supplier Product No.: 200-301-h62

Product Details anti-Caspase 1 Antibody

Target: Caspase 1 (CASP1)

Binding Specificity: C-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Caspase 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Supplier Product No.: 200-301-h62

Supplier: Rockland

Purpose: Caspase-1 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-Caspase-1 from human and mouse based on 100 % homology with the immunizing sequence.

Purification: Anti-Caspase-1 Antibody was purified by Protein G chromatography.

Immunogen: Caspase-1 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to amino acids near the c-terminus of the immunogen, human Caspase-1.

Clone: 14F468

Isotype: IgG1 kappa

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:100 - 1:500

Western_Blot_Dilution: 0.5-2 μg/mL

Comment:

Anti-Caspase-1 antibody has been tested in IHC-F, IHC-P, IF, and WB. Expect a band approximately 46kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Caspase 1

Target: Caspase 1 (CASP1)

Alternative Name: Caspase-1 (CASP1 Products)

Background:

Synonyms: IL1BC, IL1BCE, Caspase-1, CASP-1, 3.4.22.36, Interleukin-1 beta convertase, IL-1BC, Interleukin-1 beta-converting enzyme, ICE, IL-1 beta-converting enzyme, p45, Caspase-1 subunit p20, Caspase-1 subunit p10

Background: Anti-Caspase-1 detects human Caspase. Caspase proteins are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspase proteins is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into tetramer to form the active enzyme. Caspase-1 (ICE, IL-1b converting enzyme) is similar to the cell death gene CED-3 of Caenorhabditilis elegans and regulates multiple proinflammatory cytokines, including interleukin-1b and interferon-gamma-inducing factor. Anti-Caspase-1 antibody is ideal for investigators involved in apoptosis research.

Gene Name: CASP1

Gene ID: 834

NCBI Accession: NP_001214

UniProt: P29466

Pathways: Apoptosis, Interferon-gamma Pathway, Positive Regulation of Endopeptidase Activity, Inflammasome