LOX antibody

Catalog No. ABIN6655735

Supplier Product No.: 100-401-h91

Product Details anti-LOX Antibody

Target: LOX (Lysyl Oxidase (LOX))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LOX antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Supplier Product No.: 100-401-h91

Supplier: Rockland

Purpose: Lysyl Oxidase Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-Lysyl Oxidase from human, bovine, chimpanzee, mouse and rat 100 % homology with the immunizing sequence.

Purification: Anti-Lysyl Oxidase Antibody is crude rabbit sera.

Immunogen: Lysyl Oxidase Antibody was produced from whole rabbit serum prepared by repeated immunizations with the human protein Lysyl Oxidase at an internal amino acid sequence.

Isotype: IgG

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: 1:100

IF_Microscopy_Dilution: User Optimized

Western_Blot_Dilution: 1:250-1:1000

Comment:

Anti-Lysyl Oxidase antibody has been tested in WB, ICC/IF, IHC-F, IHC-P, IP. Expect a band approximately 31kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Optional[Buffer]: 50 % (v/v) Glycerol ,0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for LOX

Target: LOX (Lysyl Oxidase (LOX))

Alternative Name: Lysyl Oxidase (LOX Products)

Background:

Synonyms: Lysyl oxidase, LOX, Protein-lysine-6 oxidase

Background: Anti-Lysyl Oxidase antibody detects human Lysyl Oxidase. Lysyl Oxidase (LOX) belongs to a heterogeneous family of enzymes that oxidize primary amine substrates to reactive aldehydes. It plays a vital role in the formation and repair of ECM. Localized both in the cytosol and nucleus, it catalyzes the oxidation and deamination of epsilon-NH2 group of lysine and hydroxylysine residues to alpha-aminoadipic-delta-semialdehyde. This is the first step that initiates the covalent cross-linking of collagen and tropoelastin resulting in the formation of insoluble collagen and elastic fibers in the extracellular matrix. LOX is a multifunctional enzyme having diverse biological functions such as developmental regulation, tumor suppression, cell motility and cellular senescence. Deficiency of LOX is seen in certain carcinomas and 3X-linked recessive human connective tissue disorders-Cutis Laxa. Anti-Lysyl Oxidase antibody is ideal for investigators involved in cytokines and growth factors.

Gene Name: LOX

Gene ID: 4015

NCBI Accession: NP_002308

UniProt: P28300

Pathways: SARS-CoV-2 Protein Interactome