FKBP4 antibody

Catalog No. ABIN6656074

Supplier Product No.: 200-301-f29

The Mouse Monoclonal anti-FKBP4 antibody has been validated for WB, IHC, IP and IF. It is suitable to detect FKBP4 in samples from Human.

Quick Overview for FKBP4 antibody (ABIN6656074)

Target: FKBP4 (FK506 Binding Protein 4, 59kDa (FKBP4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FKBP4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: Hi52C

Product Details anti-FKBP4 Antibody

Supplier Product No.: 200-301-f29

Supplier: Rockland

Purpose: FKBP52 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with FKBP52 from Canine, Hamster, Human, Mouse, and Rat based on 100 % homology with the immunizing sequence.

Purification: Anti-FKBP52 Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: FKBP51 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to the residues of human FKBP51.

Isotype: IgG

Application Details

Application Notes:

Immunoprecipitation_Dilution: 5 μg/20uL

Immunohistochemistry_Dilution: 1:250

Western_Blot_Dilution: 1:2000

Comment:

Anti-FKBP52 Antibody is tested for WB, IP, IF, and IHC. Expect a band approximately ~52kDa protein representing FKBP52 in cell lysates. Heavy chain migrates close to FKBP52 on SDS PAGE. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for FKBP4

Target: FKBP4 (FK506 Binding Protein 4, 59kDa (FKBP4))

Alternative Name: FKBP52

Background:

Synonyms: FK506 binding protein 4, FKBP4, FKBP59, HBI, Hsp56, p52, p59, PPIase, Rotamase, T cell FK506 binding protein, FKBP52

Background: Hsp90 is crucial to cellular signaling by its regulation of the folding, activity, and stability of a wide range of client proteins. These client protein complexes may also contain one or more cochaperones. One class of Hsp90-binding cochaperone is composed of proteins with a characteristic tetratricopeptide repeat (TPR) domain that forms an Hsp90 binding site. Among the TPR cochaperones of Hsp90 are Hop/Sti1, protein phosphatase PP5, and members of both the FK506- and cyclosporin A-binding families of immunophilins. FK506-binding protein 51 (FKBP51) and FKBP52 are large molecular weight immunophilins that are part of the mature glucocorticoid receptor (GR) heterocomplex. The N terminal domain of each protein binds FK506 and has peptidyl-prolyl isomerase (PPIase) activity that converts prolyl peptide bonds within target proteins from cis- to trans- proline. The C-terminal domains contain the TPR repeats involved in protein-protein interactions with the Hsp90. Although FKBP52 and FKBP51 share ~75 % sequence similarity, they affect hormone binding by glucocorticoid receptor in opposing manners and have different Hsp90-binding characteristics . FK506 binding protein 51 kDa (FKBP51 or otherwise referred to as FKBP54) has been identified as a progestininducible gene. This protein is predominantly expressed in murine T cells but in humans, it is abundantly expressed in numerous tissues at levels many times higher than FKBP12. The FKBP51 gene is known to be induced by glucocorticoids.

Gene Name: FKBP4

Gene ID: 2288

NCBI Accession: NP_002005

UniProt: Q02790

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway