WASF1 antibody (C-Term)

Catalog No. ABIN6656415

Supplier Product No.: 200-301-g64

Product Details anti-WASF1 Antibody

Target: WASF1 (WAS Protein Family, Member 1 (WASF1))

Binding Specificity: C-Term

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This WASF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM), Immunoprecipitation (IP)

Supplier Product No.: 200-301-g64

Supplier: Rockland

Purpose: WAVE1-Scar Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with WAVE1-Scar from Mouse, Human, and Rat based on 100 % homology with the immunizing sequence.

Purification: Anti-WAVE1-Scar Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: WAVE1-Scar Antibody was produced in mice by repeated immunizations raised against a synthetic peptide corresponding to the near c-terminal region of human Wave1/Wasp family1.

Clone: S91-36

Isotype: IgG1

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: 0.1-1.0 μg/mL

IF_Microscopy_Dilution: 1.0-10 μg/mL

Western_Blot_Dilution: 1 μg/mL

Comment:

Anti-WAVE1-Scar Antibody is suitable for use in WB, IHC and IP. Expect a band approximately 75kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for WASF1

Target: WASF1 (WAS Protein Family, Member 1 (WASF1))

Alternative Name: WAVE1-Scar (WASF1 Products)

Background:

Synonyms: Wave1, Scar1, WASP, Wave, WASF1, Wiskott-Aldrich syndrome protein family member 1, WASP family protein member 1, Verprolin homology domain-containing protein 1

Background: The Wiskott-Aldrich syndrome protein (WASP) family plays essential roles in the regulation of actin polymerization. There are five WASP family members: Wasp, N-WASP, Wave1 (WASP family verprolin-homologous protein 1), WAVE2 and WAVE3. The Wasp family proteins share a conserved C-terminal region called the verprolin homology, cofilin homology and acidic domain. The Wave/Scar family of prtoteins mediates signals to actin assembly by direct activation of the Arp2/3 complex. These proteins have been characterized as major regulators of lamellipodia formation downstream of Rac activation, and as members of large protein complexes. IT has been found that all three Scar/Wave isoforms behave similarily and are likely to participate in the same kinds of protein complexes.

Gene Name: WASF1

Gene ID: 8936

NCBI Accession: NP_001020105

UniProt: Q92558

Pathways: RTK Signaling, Regulation of Actin Filament Polymerization