WASF1 antibody (pTyr125)

Catalog No. ABIN1531413

This Rabbit Polyclonal antibody specifically detects WASF1 in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for WASF1 antibody (pTyr125) (ABIN1531413)

Target: WASF1 (WAS Protein Family, Member 1 (WASF1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WASF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-WASF1 Antibody

Binding Specificity: AA 91-140, pTyr125

Purpose: WAVE1 (phospho Tyr125) Antibody

Specificity: Phospho-WAVE1 (Y125) Polyclonal Antibody detects endogenous levels of WAVE1 protein only when phosphorylated at Y125.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human WAVE1 around the phosphorylation site of Tyr125. AA range:91-140

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for WASF1

Target: WASF1 (WAS Protein Family, Member 1 (WASF1))

Alternative Name: WAVE1

Background:

Synonyms: WASF1, KIAA0269, SCAR1, WAVE1, Wiskott-Aldrich syndrome protein family member 1, WASP family protein member 1, Protein WAVE-1, Verprolin homology domain-containing protein 1

Background: The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008],

Gene Name: WASF1

Protein Name: Wiskott-Aldrich syndrome protein family member 1

Molecular Weight: 61 kDa

Gene ID: 8936

OMIM: 605035

UniProt: Q92558

Pathways: RTK Signaling, Regulation of Actin Filament Polymerization