CNTNAP2 antibody (Extracellular Domain)

Catalog No. ABIN6656679

Supplier Product No.: 200-301-f04

This Mouse Monoclonal antibody specifically detects CNTNAP2 in WB, IHC, IP and FM. It exhibits reactivity toward Human.

Quick Overview for CNTNAP2 antibody (Extracellular Domain) (ABIN6656679)

Target: CNTNAP2 (Contactin Associated Protein-Like 2 (CNTNAP2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CNTNAP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Clone: S67-25

Product Details anti-CNTNAP2 Antibody

Binding Specificity: Extracellular Domain

Supplier Product No.: 200-301-f04

Supplier: Rockland

Purpose: CASPR2 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with CASPR2 from Mouse, Human, and Rat based on 100 % homology with the immunizing sequence.

Purification: Anti-CASPR2 Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: CASPR2 Antibody was produced in mice by repeated immunizations with a fusion protein (extracellular domain) of human CASPR2.

Isotype: IgG2a

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: 0.1-1.0 μg/mL

IF_Microscopy_Dilution: 1.0-10 μg/mL

Western_Blot_Dilution: 1 μg/mL

Comment:

Anti-CASPR2 Antibody is tested in WB, IP, and IHC. Expect a band approximately ~180kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 1X PBS, pH 7.4

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CNTNAP2

Target: CNTNAP2 (Contactin Associated Protein-Like 2 (CNTNAP2))

Alternative Name: CASPR2

Background:

Synonyms: Cntnap2, CNTP2, Contactin associated protein like 2, Cell recognition molecule Caspr2, CASPR2, KIAA0868

Background: Caspr (contactin-associated protein) is a part of the neurexin family. It lies in the paranodal section of the myelin sheath. It's role is for myelin sheath attachment along with contactin in a cis-complex (3). Caspr and Caspr2 regulate the formation of distinct axonal domains around the nodes of Ranvier. Caspr is required for the generation of a membrane barrier at the paranodal junction, whereas Caspr serves as a membrane scaffold that clusters Kv1 channels at the juxtaparanodal region. Both interact with protein 4.1B.

Gene Name: CNTNAP2

Gene ID: 26047

NCBI Accession: NP_054860

UniProt: Q9UHC6