Paxillin antibody (AA 305-559)

Catalog No. ABIN6657483

Supplier Product No.: 200-301-p44

Product Details anti-Paxillin Antibody

Target: Paxillin (PXN)

Binding Specificity: AA 305-559

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Paxillin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 200-301-p44

Supplier: Rockland

Purpose: Paxillin Antibody

Cross-Reactivity (Details): This product reacts with human, mouse, rat, and chicken.

Purification: Anti-Paxillin antibody was purified from mouse ascites by protein A chromatography.

Immunogen: Paxillin antibody was produced in mice by repeated immunizations with recombinant protein corresponding to the C-terminal part of chicken paxillin (amino acids 305-559).

Clone: PXC-10

Isotype: IgG1

Application Details

Application Notes:

ELISA_Dilution: 1:20,000

Western_Blot_Dilution: 1-2 μg/mL

Other: ICC: 1:100-1:500

Comment:

Anti-Paxillin is useful for Immunocytochemistry and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~64.5 kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Neutral PBS

Reconstitution_Volume: 1.0 mL

Buffer:

Buffer: 1.2 % sodium acetate

Stabilizer: 2 mg BSA

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Paxillin

Target: Paxillin (PXN)

Alternative Name: Paxillin (PXN Products)

Background:

Synonyms: Paxillin antibody, PXN

Background: The paxillin gene can be alternatively spliced to include 1 of 2 alternative exons, generating beta and gamma isoforms. Paxillin is a 68- kDa focal adhesion protein that is phosphorylated on tyrosine residues in fibroblasts in response to transformation by v-src, treatment with platelet-derived growth factor, or cross-linking of integrins. The 68-kD protein (paxillin) is a cytoskeletal component that localizes to the focal adhesions at the ends of actin stress fibers in chicken embryo fibroblasts. It is also present in the focal adhesions of Madin-Darby bovine kidney (MDBK) epithelial cells but is absent, like talin, from the cell-cell adherens junctions of these cells. This antibody is suitable for researchers interested in growth factors and signal trasduction.

Gene Name: PXN

Gene ID: 5829

NCBI Accession: NP_001074324

UniProt: P49023

Pathways: MAPK Signaling, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling