Paxillin antibody (PXN) (AA 456-472)

Details for Product anti-PXN Antibody No. ABIN3044187
Antigen
  • AW108311
  • AW123232
  • Pax
  • CG18061
  • CG18576
  • CG31794
  • CT40481
  • CT42454
  • DPaxillin
  • DPxn
  • DPxn37
  • Dmel\\CG31794
  • Dpax
  • DpaxA
  • PDLP
  • dPax
  • pax
  • wu:fw71f12
  • PXN
  • LOC100220858
  • paxillin
  • Paxillin
  • paxillin S homeolog
  • paxillin a
  • PXN
  • Pxn
  • Pax
  • pxn.S
  • pxna
  • LOC696336
  • pxn
Alternatives
anti-Human Paxillin antibody for Western Blotting
Epitope
AA 456-472, C-Term
67
65
38
30
24
17
15
13
11
10
7
6
6
6
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
458
247
230
27
12
10
8
5
5
5
4
3
1
1
1
1
Host
Rabbit
428
40
2
Clonality
Polyclonal
Conjugate
This Paxillin antibody is un-conjugated
15
13
13
13
13
13
13
6
6
4
4
4
4
4
2
2
2
2
2
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
340
129
116
53
46
46
27
18
11
8
3
2
1
Options
Purpose Rabbit IgG polyclonal antibody for Paxillin(PXN) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminal of human Paxillin(456-472aa HEKDGKAYCRKDYFDMF), identical to the related mouse and rat sequences.
Sequence HEKDGKAYCR KDYFDMF
Isotype IgG
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Paxillin(PXN) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: paxillin
Protein Name: Paxillin
Purification Immunogen affinity purified.
Plasmids, Primers & others Plasmids, Primers & others Paxillin products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name PXN (PXN Antibody Abstract)
Background PXN(Paxillin) is a signal transduction adaptor protein discovered in 1990 in the laboratory of Keith Burridge. Salgia et al.(1995) mapped the paxillin gene to 12q24 using fluorescence in situ hybridization.The C-terminal region of paxillin contains four LIM domains that target paxillin to focal adhesions, it is presumed through a direct association with the cytoplasmic tail of beta-integrin. The N-terminal region of paxillin is rich in protein-protein interaction sites. The proteins that bind to paxillin are diverse and include protein tyrosine kinases, such as Src and FAK, structural proteins, such as vinculin and actopaxin, and regulators of actin organization, such as COOL/PIX and PKL/GIT. Paxillin is tyrosine-phosphorylated by FAK and Src upon integrin engagement or growth factor stimulation, creating binding sites for the adapter protein Crk.The paxillin protein contains 4 LIM domains, a proline-rich domain containing a consensus SH3-binding site, and 3 potential SH2-binding sites. On Northern blots, paxillin was expressed as a 3.7-kb mRNA in all tissues tested.

Synonyms: FLJ16691 antibody|FLJ23042 antibody|Paired box protein Pax 1 antibody|PAX 1 antibody|PAX1 antibody|PAXI_HUMAN antibody|Paxillin alpha antibody|Paxillin antibody|PXN antibody|PXN protein antibody
UniProt P49023
Pathways MAPK Signaling, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-Paxillin (PXN) (AA 456-472), (C-Term) antibody (ABIN3044187) Anti-Paxillin antibody, Western blotting Lane 1: 293T Cell Lysate Lane 2: HELA Cell L...
Immunohistochemistry (IHC) image for anti-Paxillin (PXN) (AA 456-472), (C-Term) antibody (ABIN3044187) Anti-Paxillin antibody, IHC(F) IHC(F): Rat Kidney Tissue
Immunohistochemistry (IHC) image for anti-Paxillin (PXN) (AA 456-472), (C-Term) antibody (ABIN3044187) Anti-Paxillin antibody, IHC(P) IHC(P): Human Lung Cancer Tissue
Immunohistochemistry (IHC) image for anti-Paxillin (PXN) (AA 456-472), (C-Term) antibody (ABIN3044187) Anti-Paxillin antibody, ICC ICC: HELA Cell
Product cited in: Zhu, Liu, Li, Liu, Wang, Sun, Xiong, Jiang, Zheng, Hu: "Protein tyrosine phosphatase receptor U (PTPRU) is required for glioma growth and motility." in: Carcinogenesis, Vol. 35, Issue 8, pp. 1901-10, 2014 (PubMed).

Liu, Zhu, Xiong, Zheng, Hu, Qiu: "Knockdown of protein tyrosine phosphatase receptor U inhibits growth and motility of gastric cancer cells." in: International journal of clinical and experimental pathology, Vol. 7, Issue 9, pp. 5750-61, 2014 (PubMed).

Zhong, Luo, Wang, Tang, Wu, Wang, Huang, Sun, Huang: "Effects of polydopamine functionalized titanium dioxide nanotubes on endothelial cell and smooth muscle cell." in: Colloids and surfaces. B, Biointerfaces, Vol. 116, pp. 553-60, 2014 (PubMed).

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