Caldesmon antibody

Catalog No. ABIN6657487

Supplier Product No.: 200-301-p24

The Mouse Monoclonal anti-Caldesmon antibody has been validated for WB, IHC and ELISA. It is suitable to detect Caldesmon in samples from Human.

Quick Overview for Caldesmon antibody (ABIN6657487)

Target: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Caldesmon antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: HHCD

Product Details anti-Caldesmon Antibody

Supplier Product No.: 200-301-p24

Supplier: Rockland

Purpose: Caldesmon (smooth) Antibody

Cross-Reactivity (Details): This product reacts with human, mouse, and rabbit.

Purification: Anti-Caldesmon antibody was purified from mouse ascites by protein A chromatography.

Sterility: Sterile filtered

Immunogen: Caldesmon (smooth) antibody was produced in mice by repeated immunizations with human uterus smooth muscle extract.

Isotype: IgG1

Application Details

Application Notes:

ELISA_Dilution: 1:20,000

Immunohistochemistry_Dilution: 1:100-1:500

Western_Blot_Dilution: 1-2 μg/mL

Comment:

Anti-Caldesmon is tested for Immunohistochemistry -P and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~93.2 kDa with isoforms ~60kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 1.2 % sodium acetate

Stabilizer: 2 mg BSA

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Caldesmon

Target: Caldesmon (CALD1) (Caldesmon 1 (CALD1))

Alternative Name: Caldesmon (smooth)

Background:

Synonyms: CDM, H-CAD, L-CAD

Background: Caldesmon is a potential actomyosin regulatory protein found in smooth muscle and nonmuscle cells. The predicted smooth-muscle polypeptide is 793 amino acids long. The high molecular weight caldesmon (h-CaD) is predominantly expressed in smooth muscles, whereas the low molecular weight caldesmon (l-CaD) is widely distributed in nonmuscle tissues and cells. Hayashi et al. (1992) demonstrated that the human CDM gene is composed of 14 exons. This antibody is suitable for researchers interested in neurodegenerative diseases, developmental signaling, GPCR signaling, and MAK Kinase Signaling.

Gene Name: CALD1

Gene ID: 800

NCBI Accession: NP_004333

UniProt: Q05682

Pathways: Myometrial Relaxation and Contraction