MUC5B antibody

Catalog No. ABIN6657491

Supplier Product No.: 200-301-p41

This anti-MUC5B antibody is a Mouse Monoclonal antibody detecting MUC5B in WB, IHC and ELISA. Suitable for Human.

Quick Overview for MUC5B antibody (ABIN6657491)

Target: MUC5B (Mucin 5 Subtype B (MUC5B))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MUC5B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 45M1

Product Details anti-MUC5B Antibody

Supplier Product No.: 200-301-p41

Supplier: Rockland

Purpose: Mucin Gastric Antibody

Cross-Reactivity (Details): This product reacts with human, mouse, rat, and rabbit.

Purification: Anti-Mucin Gastric antibody was purified from mouse ascites by protein A chromatography.

Immunogen: Mucin Gastric antibody was produced in mice by repeated immunizations with mucin from human ovarian cyst fluid.

Isotype: IgG1

Application Details

Application Notes:

ELISA_Dilution: 1:20,000

Immunohistochemistry_Dilution: 2-4 μg/mL

Western_Blot_Dilution: 1-2 μg/mL

Other: ICC: 1 μg/mL

Comment:

Anti-Mucin Gastric is tested for Immunocytochemistry, Immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~596 kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Neutral PBS

Reconstitution_Volume: 1.0 mL

Buffer:

Buffer: 1.2 % sodium acetate

Stabilizer: 2 mg BSA

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for MUC5B

Target: MUC5B (Mucin 5 Subtype B (MUC5B))

Alternative Name: MUC5B

Background:

Synonyms: MG1, MUC5, MUC9, MUC-5B, Cervical mucin, High molecular weight salivary mucin MG1, Mucin-5 subtype B, tracheobronchial, Sublingual gland mucin

Background: MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed abundantly in >90 % breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. This antibody is suitable for researchers interested in neuroscience research, developmental signaling, and cell adheshion.

Gene Name: MUC5B

Gene ID: 727897

NCBI Accession: NP_002449

UniProt: Q9HC84