MMP16 antibody (C-Term)

Catalog No. ABIN6657532

Supplier Product No.: 600-401-p76

The Rabbit Polyclonal anti-MMP16 antibody has been validated for WB and IHC. It is suitable to detect MMP16 in samples from Human.

Quick Overview for MMP16 antibody (C-Term) (ABIN6657532)

Target: MMP16 (Matrix Metallopeptidase 16 (Membrane-inserted) (MMP16))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MMP16 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-MMP16 Antibody

Binding Specificity: C-Term

Supplier Product No.: 600-401-p76

Supplier: Rockland

Purpose: MMP16 Antibody

Cross-Reactivity (Details): Anti-MMP16 antibody is directed against human MMP16 protein.

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: MMP16 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a sequence at the C-terminal of human MMP16.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:100-1:500

Western_Blot_Dilution: 0.5 μg/mL

Comment:

Anti-MMP16 is tested for Immunohistochemistry-P, -F, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~69.5 kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide

Stabilizer: 5 mg BSA

Preservative: 0.05 mg Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for MMP16

Target: MMP16 (Matrix Metallopeptidase 16 (Membrane-inserted) (MMP16))

Alternative Name: MMP16

Background:

Synonyms: MMP-X2,Membrane-type matrix metalloproteinase 3,

Background: The matrix metalloproteinase 16(MMP16) protein consists of 604 amino acids and has a characteristic MMP domain structure, which gene is mapped on human chromosome 8q21. Additionally, MMP16 has a C-terminal extension containing a potential transmembrane domain, similar to MMP14 , MMP15 , and MMP17. Furthermore, it is membrane-bound and is a member of the membrane-type MMPs that are a subclass in the MMP family since the other members lack a C-terminal transmembrane domain and are secreted as soluble forms. MMP16 is expressed as a 12-kb transcript in brain, placenta, heart, and some carcinoma cell lines, but is not detectably expressed in lung, kidney, liver, spleen, and muscle. This antibody is suitable for researchers interested in cancer research.

Gene Name: MMP16

Gene ID: 4325

NCBI Accession: NP_005932

UniProt: P51512