Involucrin antibody

Catalog No. ABIN6657536

Supplier Product No.: 200-301-p39

This anti-Involucrin antibody is a Mouse Monoclonal antibody detecting Involucrin in WB, IHC and ELISA. Suitable for Human.

Quick Overview for Involucrin antibody (ABIN6657536)

Target: Involucrin (IVL)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Involucrin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: SY5

Product Details anti-Involucrin Antibody

Supplier Product No.: 200-301-p39

Supplier: Rockland

Purpose: Involucrin Antibody

Cross-Reactivity (Details): This product reacts with human and pig.

Purification: Anti-Involucrin antibody was purified from mouse ascites by protein A chromatography.

Immunogen: Involucrin antibody was produced in mice by repeated immunizations with human involucrin.

Isotype: IgG1

Application Details

Application Notes:

ELISA_Dilution: 1:20,000

Immunohistochemistry_Dilution: 1:100-1:500

Western_Blot_Dilution: 1 μg/mL

Other: Immunocytochemistry: 1 μg/mL

Comment:

Anti-Involucrin is tested for Immunohistochemistry -P, -F, Immunocytochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~68.5 kDa corresponding to the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Neutral PBS

Reconstitution_Volume: 1.0 mL

Buffer:

Buffer: 1.2 % sodium acetate

Stabilizer: 2 mg BSA

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Involucrin

Target: Involucrin (IVL)

Alternative Name: Involucrin

Background:

Synonyms: INVO, IVL, Involucrin Antibody

Background: Involucrin is a keratinocyte protein that first appears in the cell cytosol, but ultimately becomes cross-linked to membrane proteins by transglutaminase. The gene consists of 585 amino acids, 390 of which form a central decapeptide repeat, rich in glutamine and glutamic acid. The involucrin gene, encoding a protein of the terminally differentiated keratinocyte, is polymorphic in the human. Involucrin has previously been mapped to chromosome 1q21. The epidermal protein involucrin has been remodeled in the higher primates. And the existence of two populations of East Asian involucrin alleles gives support for the existence of a Eurasian stem lineage from which Caucasians and a part of the Asian population originated. This antibody is suitable for researchers interested in skin cell research.

Gene Name: IVL

Gene ID: 3713

NCBI Accession: NP_005538

UniProt: P07476