GLI1 antibody (AA 805-820)

Catalog No. ABIN6657595

Supplier Product No.: 100-401-223s

The Rabbit Polyclonal anti-GLI1 antibody has been validated for WB, ELISA, IF and FM. It is suitable to detect GLI1 in samples from Mouse. There are 8+ publications available.

Quick Overview for GLI1 antibody (AA 805-820) (ABIN6657595)

Target: GLI1 (Zinc Finger Protein GLI1 (GLI1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLI1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Product Details anti-GLI1 Antibody

Binding Specificity: AA 805-820

Supplier Product No.: 100-401-223s

Supplier: Rockland

Purpose: Gli1 Antibody

Cross-Reactivity (Details): Reactivity is observed against Mouse and Human Gli-1.

Purification: This whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative.

Sterility: Sterile filtered

Immunogen: The whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acids 805-820 of mouse Gli-1. The peptide was synthesized as a multiple antigen peptide (MAP).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:20,000 - 1:100,000

IF_Microscopy_Dilution: 1:500 - 1:2,000

Western_Blot_Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Comment:

Suggested Applications: ChIP, IHC, IP
This antibody has been tested for use in ELISA, Immunofluorescence, and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 120 kDa in size corresponding to Gli-1 protein by western blotting in the appropriate cell lysate or extract. For immunohistochemistry, perform heat mediated antigen retrieval via the microwave method before commencing with staining.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

References for anti-GLI1 antibody (ABIN6657595)

Takeuchi, Kito, Itoh, Naruse, Fujikawa, Sadek, Akiyama, Yamashiro, Wakisaka, Abe: "Kruppel-Like Factor 4 represses osteoblast differentiation via ciliary Hedgehog signaling." in: Experimental cell research, Vol. 371, Issue 2, pp. 417-425, (2018) (PubMed).

Armas-López, Piña-Sánchez, Arrieta, de Alba, Ortiz-Quintero, Santillán-Doherty, Christiani, Zúñiga, Ávila-Moreno: "Epigenomic study identifies a novel mesenchyme homeobox2-GLI1 transcription axis involved in cancer drug resistance, overall survival and therapy prognosis in lung cancer patients." in: Oncotarget, Vol. 8, Issue 40, pp. 67056-67081, (2017) (PubMed).

Yoon, Lamm, Iannaccone, Higashiyama, Leong, Iannaccone, Walterhouse: "p53 modulates the activity of the GLI1 oncogene through interactions with the shared coactivator TAF9." in: DNA repair, Vol. 34, pp. 9-17, (2016) (PubMed).

Kotula, Sun, Li, Pratico, Fereshteh, Ahrens, Sullenger, Kovacs: "Targeted disruption of β-arrestin 2-mediated signaling pathways by aptamer chimeras leads to inhibition of leukemic cell growth." in: PLoS ONE, Vol. 9, Issue 4, pp. e93441, (2015) (PubMed).

Gurung, Feng, Hua: "Menin directly represses Gli1 expression independent of canonical Hedgehog signaling." in: Molecular cancer research : MCR, Vol. 11, Issue 10, pp. 1215-22, (2014) (PubMed).

Jalili, Mertz, Romanov, Wagner, Kalthoff, Stuetz, Pathria, Gschaider, Stingl, Wagner: "NVP-LDE225, a potent and selective SMOOTHENED antagonist reduces melanoma growth in vitro and in vivo." in: PLoS ONE, Vol. 8, Issue 7, pp. e69064, (2013) (PubMed).

Maliniemi, Carlsson, Kaukola, Ovaska, Niiranen, Saksela, Jeskanen, Hautaniemi, Ranki: "NAV3 copy number changes and target genes in basal and squamous cell cancers." in: Experimental dermatology, Vol. 20, Issue 11, pp. 926-31, (2012) (PubMed).

Yoon, Gilbertson, Iannaccone, Iannaccone, Walterhouse: "Defining a role for Sonic hedgehog pathway activation in desmoplastic medulloblastoma by identifying GLI1 target genes." in: International journal of cancer, Vol. 124, Issue 1, pp. 109-19, (2009) (PubMed).

Target Details for GLI1

Target: GLI1 (Zinc Finger Protein GLI1 (GLI1))

Alternative Name: Gli1

Background:

Synonyms: rabbit anti-Gli-1 Antibody, rabbit anti-Gli1 Antibody, Zinc finger protein GLI1 antibody, Glioma-associated oncogene antibody, Oncogene GLI antibody

Background: Anti Gli1 Antibody was produced against a peptide corresponding to the carboxy-terminal region of the mouse Gli-1 protein. This region of Gli1 is not conserved among other gli family members, namely Gli-2 and Gli-3. Gli was termed by Kinzler et al. (1987) as 'glioma-associated oncogene' amplified in malignant gliomas. Analysis of the cloned gene demonstrates that the gene contains 5 repeats of zinc-finger sequences, which places Gli in the family of Kruppel (Kr) zinc finger proteins. Northern analysis reveals that Gli is expressed in embryonal carcinoma cells but not in most adult tissue. Gli has been localized to 12q13-q14.3 by Southern blot analysis. In mice, the gene is located on chromosome 10. In mice, three zinc finger transcription factors, Gli-1, Gli-2 and Gli-3, have been implicated in the transduction of Sonic hedgehog (Shh) signal. In papillary epithelium, shh, gli1 and ptc all follow similar expression patterns. Gli-1 expression is central and probably sufficient for tumor development in humans.

Gene Name: GLI1

Gene ID: 14632, 4885279

UniProt: P47806

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis